EFFECT OF ETHANOLIC EXTRACT OF MOMORDICA CHARANTIA ON ANTIOXIDANT AND INFLAMMATORY MARKERS IN MALE BALB/c MICE ADMINISTERED ACYCOR-PLUS
Bimpe Ogungbe1, Michael Arowosegbe2, Green Akenzua3, Olaide Elewuro4, and Semako Hungbo5
1Biochemistry Department, Faculty Of Science, Lagos State University, Ojo, Lagos Nigeria., Nigeria, 2Biochemistry Department, Lagos State University., Nigeria, 3Biochemistry Department, Faculty Of Science, Lagos State University, Ojo, Lagos Nigeria., Nigeria, 4Biochemistry Department, Faculty Of Science, Lagos State University, Ojo, Lagos Nigeria., Nigeria, and 5Biochemistry Department, Faculty Of Science, Lagos State University, Ojo, Lagos Nigeria., Nigeria
Introduction: Acycor-plus (ACY) is a single dose combined analgesic and anti-inflammatory drug. Prolonged usage and overdose often lead to gastro-intestinal ulcerations, inflammations and oxidative stress which increases the use of synthetic H2 antagonists such as cimetidine (CMT).
Aims: This study was carried out to monitor the antioxidant and inflammatory effects of ethanolic extract of Momordica charantia (MC) in ACY-administered male Balb/c mice.
Materials and Methods: Twenty-five mice weighing between 15-20g were equally divided into five groups and received normal saline (Control), 2.5mg/kg ACY alone (ACY), ACY combined with either 50mg/kg cimetidine (CMT) or 100mg/kg and 200mg/kg ethanolic extract of Momordica charantia (MC100 and MC200, respectively). The animals were sacrificed after three days by cervical dislocation and post-mitochondrial fractions of their livers, kidneys, small intestines and colons were used to assess the activities of antioxidant and inflammatory markers.
Results: MC100 and MC200 significantly reduced antioxidant markers except catalase (CAT) when compared with ACY while they increased hepatic myeloperoxidase (MPO) and nitric oxide (NO) (p<0.05). Kidney and colonic MPO activities were significantly reduced by MC100. However, MC200 significantly increased renal CAT, superoxide dismutase (SOD) and SOD/(GPx+CAT) ratio with increased oxidative stress.
Conclusion: It can be concluded that MC100 had comparable effect with CMT while MC200 might induce renal toxicity. However, further research is needed using other routes of administration.
Ulceration, Inflammations, Oxidative stress, and Momordica charantia