Volume 11, Issue 1
Investigation of Bacterial Organisms Associated with Eggs of Cultured Broodstock Catfish Clarias gariepinus (Burchell, 1822) from Ogun State, Nigeria
Folalu AWE1, Gabriel MEKULEYI 2, BADMOS Lateef3, Hammed SHITTU3,
Department of Fisheries, Faculty of Science, Lagos State University, Ojo, Lagos State, Nigeria1,
Department of Microbiology, Faculty of Science, Lagos State University, Ojo, Lagos State, Nigeria2,
Department of Fisheries, Faculty of Science, Lagos State University, Ojo, Lagos State, Nigeria3
DOI:NIL
Abstract
Introduction: The gene bank on fish diseases is usually enriched via information on the existing and emerging microorganisms. Aim: This study evaluated the bacterial organisms associated with eggs of cultured Catfish broodstock collected from a private Fish Farm in Ogun State, Nigeria. Materials and Methods: Fish samples were taken to the Microbiology laboratory, Federal University of Agriculture, Abeokuta Ogun State, for egg microbial analyses. The amplicon was taken to International Institute of Tropical Agriculture, Ibadan, for molecular characterization while Bio-edit was used for importing and mining nucleotide sequences into gene bank. Results: The results revealed that the following bacterial organisms were present in the eggs of Clarias gariepinus: Pseudomonas stutzeri, Acinetobacter gerneri, Enterobacter cloacae, Acinetobacter haemolyticus and Aeromonas caviae. Based on the similarity percentage of sequence generated and matched on NCBI database, Enterobacter cloacae had 98.36% similarity, with Acinetobacter haemolyticus of 97.21%, and Pseudomonas stutzeri with 80.48%. Acinetobacter gerneri and Aeromonas caviae had 88.08% and 93.75% similarity percentage respectively. Conclusion: Bacteria were found in the eggs of cultured catfish, although some of them are free-living water bacteria and may not pose any threat.