Introduction: The gene bank on fish diseases is usually enriched
via information on the existing and emerging microorganisms.
Aim: This study evaluated the bacterial organisms associated with
eggs of cultured Catfish broodstock collected from a private Fish
Farm in Ogun State, Nigeria.
Materials and Methods: Fish samples were taken to the
Microbiology laboratory, Federal University of Agriculture, Abeokuta
Ogun State, for egg microbial analyses. The amplicon was taken to
International Institute of Tropical Agriculture, Ibadan, for molecular
characterization while Bio-edit was used for importing and mining
nucleotide sequences into gene bank.
Results: The results revealed that the following bacterial organisms
were present in the eggs of Clarias gariepinus: Pseudomonas
stutzeri, Acinetobacter gerneri, Enterobacter cloacae, Acinetobacter
haemolyticus and Aeromonas caviae. Based on the similarity
percentage of sequence generated and matched on NCBI
database, Enterobacter cloacae had 98.36% similarity, with
Acinetobacter haemolyticus of 97.21%, and Pseudomonas stutzeri
with 80.48%. Acinetobacter gerneri and Aeromonas caviae had
88.08% and 93.75% similarity percentage respectively.
Conclusion: Bacteria were found in the eggs of cultured catfish,
although some of them are free-living water bacteria and may not
pose any threat.