Introduction: Mushrooms have been broadly used in folk medicine for the treatment of various diseases for years. Polypores are cosmopolitan mushrooms that are widely investigated for their useful properties in battling multidrug-resistant pathogens. Aims: This study investigated the phytochemical, antioxidant and antibacterial properties of wild Bondarzewia berkeleyi and Ganoderma lucidum. The fruiting bodies of G. lucidum and B. berkeleyi were collected at Lagos State University, Ojo Campus. Materials and Methods: Extraction was done using methanol and acetone. Standard tests were carried out to detect different phytochemical compounds present in the mushroom extracts. These phytochemical compounds were further estimated and quantified using standard methods. The antioxidant activity of the extracts was evaluated using a DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging assay, while antimicrobial activity on Pseudomonas aeruginosa and Salmonella typhi was carried out using the paper disc diffusion assay. Results: Results showed that reducing sugar, terpenoids, steroids, phenolics, flavonoids and triterpenoids were present in both mushrooms while tannins, alkaloids and anthraquinones were absent. Saponins appeared to be present only in the methanolic extracts, while cardiac glycosides were detected only in the acetone extract of both mushrooms. The highest antioxidant activity was recorded in the acetone extract of G. lucidum (90.435±0.112) and the least was recorded in the acetone extract of B. berkeleyi (26.632±0.129), while both mushroom extracts showed antibacterial effects against both tested organisms. The highest inhibition zone was exhibited by the methanolic extract of G. lucidum against P. aeruginosa ranging from 10 mm to 14 mm while the least inhibition zone ranging from 0 mm to 11 mm was exhibited by S. typhi for the extracts. Nevertheless, P. aeruginosa appeared to be more sensitive to G. lucidum extract than S. typhi, which exhibited the smallest zones of inhibition. Conclusion: Based on this research result, these mushroom extracts are a good source of phytochemicals that show potential for antibacterial and antioxidant activity; therefore, they can be exploited as therapeutic products.