Volume 10, Issue 2
Jatropha tanjorensis extracts mediated silver nanoparticles synthesis for possible antidiabetic, antiglycation, antioxidant and anti-inflammatory applications
Olasunkanmi Kayode Awote1*, Adesegun Gideon Adeyemo1, Sodiq Kolawole Apete1, Rasaq Bamidele Awosemo2, Habeebah Doyin Azeez1, Daniel Segun Salako1, Babajide David Kayode3, Sheu Oluwatobiloba Arowolo1, Oluwatobi Smith Olatunbosun1, Funmilayo Esther Olalero1
1Department of Biochemistry, Faculty of Science, Lagos State University, Nigeria
2Department of Cellular and Molecular Biology, University of New Haven, West haven, Connecticut, USA.
3Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Eko University of Medicine and Health Sciences, Badagry Express way, Lagos State, Nigeria.
DOI:10.36108/jrrslasu/3202.01.0180
Abstract
Introduction: The use of plant-mediated nanotechnology is gaining attention worldwide because of its low-cost and high efficiency in the synthesis of nano-sized particles that ranges between 1-100 nm. Aims: This study investigated the antidiabetic, antiglycation, antioxidant, and anti-inflammatory potentials of synthesized silver nanoparticles (AgNPs) using Jatropha tanjorensis leaf and stem extracts. Materials and Methods: AgNPs was synthesized using the aqueous extract of J. tanjorensis leaf and stem, respectively. The synthesized AgNPs were characterized using UV-Visible spectroscopy, Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The anti-diabetic (α-amylase and α–glucosidase inhibitory assays), antiglycation (fructosamine inhibition), antioxidant (reducing power, total antioxidant capacity, DPPH and NO radical scavenging assays), and anti-inflammatory (proteinase inhibitory action and albumin denaturation inhibition) activities were evaluated using standard procedures. Results: The synthesized AgNPs showed a maximum absorption peak at 410nm and 412nm, and an average size distribution of 42.66nm and 48.33nm using the leaf and stem extracts of the plant, respectively. The synthesized AgNPs using the leaf extract (JTL-AgNPs) showed a better antidiabetic, antiglycation, anti-inflammatory and free radical scavenging potentials which may possibly be due to the compounds adsorbed on the surface of the synthesized AgNPs as revealed by the FTIR analysis. Conclusion: The aqueous leaf and stem extracts of Jatropha tanjorensis can be used to synthesize AgNPs, however, the JTL-AgNPs showed better potentials on investigated parameters, thus suggesting its exploration in the development of drugs for the treatment and management of diabetes mellitus and other associated free radical causing diseases.